Madeira Chemistry Research Centre (CQM) receveid Dr. Annika Koch (Bruker Corporation) for two lectures of "MALDI-TOF MS APPLICATIONS", where was made an introduction to the techniques, potentialities and applications of this tool, followed by a practical part.
Madeira, Portugal, September 21-25, 2009
CS Madeira Atlantic Resort & Sea SPA
INEB – Instituto de Engenharia Biomédica, University of Porto
CQM - Centro de Química da Madeira, University of Madeira
An initiative part of the Work Package 4 on Education, of the BRIDGE Project.
This Course is designed for PhD students that have enrolled in the Bridge Educational Programme.
The Course will consist in 5 full days at a single location, based on 2-3 lectures per day given by internationally reputed speakers on several topics of Biomaterials Science and Regenerative Medicine. Each lecture will provide background concepts, as well as advanced topics.
The activities in this Course are interconnected with previous activities of this Educational Programme. Bridge PhD students, organized in groups, will present a Business Plan drafted during the Winter Course (Budapest, February 2009), aimed at valuing the results of research in this scientific field. In addition, students will also be given the opportunity to present their work in a Poster Session and develop a Research Project during the week, in the format of a grant application, which will be discussed and commented by the lecturers.
Overall, the added value of this Course to PhD students relies on:
The Atomic Force Microscopy Nanosurf FlexAFM System of the Centro de Química da Madeira (CQM, University of Madeira), is the first AFM equipment in Autonomous Region of Madeira, Portugal. This equipment is intended for research and has as its main priority the activities/research within the scope of the ECOFIBRAS Project (MAC / 4.6D / 040) for the topographic characterization of fibers of invasive species of Macaronesia, and as part of the research activities of CQM, the study of polymer fibers and DNA films. In addition, and depending on the availability of time, the AFM equipment is open to external services (other public or private institutions).
Atomic force microscopy consists of a type of high resolution microscopy at nanoscale. This technique is used for the analysis of surfaces, in order to obtain information such as topography (roughness, size), material contrast, electrical, magnetic and nanomechanical properties, and surface modification such as lithography. These analyzes can be performed on fixed samples (including biological samples), in air or in liquid medium. The principle of this technique is based on a small probe with a tip (ideally made up of a single atom) coupled to piezoelectric elements with the ability to scan the surface. During this scan, the sensed interactions between the tip and the sample surface atoms are translated into probe deflection / amplitude / frequency variations, which depend on the tip-to-sample distance, that is, whether the interactions are attractive or repulsive. These small variations are then converted, for example, into image, force maps or force curves. The AFM technique has been used in many different areas, such as Biological / Materials Science and Nanomanipulation.
The Nanosurf FlexAFM combined with the C3000 controller is a fully developed research system to perform AFM analysis on its own, or be coupled to inverted microscopes for combination / complementation of results.
X/Y slope correction
Force-distance, amplitude-distance, phase-distance spectroscopy, tip current-tip voltage
Setup Wizard for spectroscopy
Sample maximum dimensions: 100 mm x 9 mm (width x height)
Manual Approach: 30 mm
Scanning area: 100 μm (XY-range)
Height: 10 μm (Z-range)
Tripod stand-alone scan head with tip scanner
Piezo-based Z-actuator, Optical Z-position sensor and Closed loop Z-control
Low noise photodiode detector (4 quadrant)
Automatic and manual on/off laser (red and near-infrared)
Approach with continuous DC-motor
Automatic self-alignment for probes and magnetic alignment of probe holder
- Operation modes:
Static Force, Dynamic Force, Phase Contrast, Magnetic Force, Electric Force, Basic Spectroscopy, Basic Lithography, Spreading resistance, Force Modulation, Espetroscopia avançada, Litografia avançada
color (3.1 MP, top view)
monochrome (1.3 MP, side view)
Simultaneous display of top/side view, 4x digital zoom in three steps (1/2/4x)
Computer HP Pro A Microtower Business
Intel i5 (8th gen) processor
500 GB disc, 4GB RAM.
Monitor/flat screen TFT de 23''
64-bit Windows 10 Professional operating system.
Nanosurf C3000 Controller software v. 3.8
-Provides all functions for the microscope operation during surface analysis, other more advanced operation modes, data analysis for further data processing..
The Scanning Probe Image Processor, SPIP(TM) v. 6.4.4
- Proessing and analysis of microscope images, extraction and analysus of image data.
- Visualization and analysis of AM data such as dimensions,, image processing, profilometric or topographic data analysis, complex image analysis.
- Analysis of force data, image and force curves, spectroscopy maps.
- Graphical vector design for lithography.
CQM's AFM Equipment
- Project Manager: João Rodrigues (PI)
- Unit Manager: Rita Castro (PhD)
- Authorized Users/researchers: Rita Castro (PhD) e Deesy Pinto (PhD)
These Guidelines are intended to be a guide to the authorised users and applicants that uses the CQM AFM equipment and as such must be followed by all. Otherwise, the access to use the microscope will be denied.
Atomic Force Microscopy (AFM) services
Guidelines for samples preparation and features
The user fees here presented are intended to cover CQM AFM expenses, which includes analysis (and normal use of consumables), technical support, parts and equipment maintenance. Minimum time of 2 hours. After 2 hours, the session will be charged in blocks of 30 minutes.
The services are classified by users and type of analysis. Choose the class that are best suited to your case or consult us at the address: .
Class 1 - FCT National Centers
Class 2 - Regional and National Public Laboratories
Class 3 - Companies and Laboratories or private Research Centers
Services charges - Price per hour (€/h)*
|AFM Analysis type||Class 1||Class 2||Class 3|
|Basic AFM||Topography (contact and non-contact mode)||21.00||41.00||50.00|
|Force Spectroscopy / Force maps||21.00||41.00||50.00|
|Special Probe||Operation in Liquid||33.00||54.00||62.00|
|High Resolution (air / liquid)||66.00||82.00||91.00|
|Mica sheets / Adhesive tapes||5.00||2.00|
|Cleaning (Solvents / Ultrasound)||5.00||5.00|
* Add tax over services with a rate of 22%
To access the Atomic Force Microscopy service, provided by CQM, follow the sample preparation guide and the regulation available above. If you are not a member of CQM and would like to do AFM reviews, please contact us at .
After submitting the form, you will receive an email in your mailbox with the indication that your registration was successful and with the filled form attached.
The Principal Investigator and CQM acknowledges for the financial support of the MAC 2014-2020 Territorial Cooperation Program through the European Regional Development Fund (FEDER) under the ECOFIBRAS Project (Sustainable exploitation of Macaronesia invasive plant species for industrial fibers, MAC/4.6 D/040).
Findo o período de contestação dos resultados provisórios, informamos que o resultado final referente ao acto Eleitoral para Coordenador Científico do Centro de Química da Madeira para o biénio 2015-2017, é o seguinte:
P´la Comissão Eleitoral
Professora Doutora Paula Castilho (Presidente)
Torna-se público o resultado provisório referente ao acto Eleitoral para Coordenador Científico do Centro de Química da Madeira para o biénio 2015-2017:
P´la Comissão Eleitoral
Professora Doutora Paula Castilho (Presidente)