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CQM Summer Internships - July 2018


Registration is open!


Registration is open for the CQM Summer Internships 2018 wich will be held during July at the CQM laboratories.

These internships will allow students to contact for the first time with the research held in scientific laboratories and are available for students of the University of Madeira (1st Cycle of Biochemistry, 2nd Cycle of Applied Biochemistry and 2nd Cycle of Nanochemistry and Nanomaterials). Students from other universities (Biochemistry, Chemistry or related areas) can also attend under the conditions indicated bellow.



Internships Proposals

PROPOSAL 1

Isolamento e caracterização de polímeros de frutose bioativos

Supervisor: Paula Castilho

Vacancies: 2

Starting date: 02/07/2018

Working time per day: 6-7h

Schedule: 09h00 – 17h00

Observation and other requirements: Minimum score of 14 in Elementary Laboratory Techniques and in Complementary Laboratory Techniques.

Abstract: Isolar e caracterizar açúcares com atividade prebiótica a partir de banana verde. Estes açúcares têm efeitos benéficos para o organismo humano na redução no teor calórico do alimento, no menor risco de formação de cáries e na redução da intolerância à lactose, e também atuando no sentido de modular o sistema imune, regular o trânsito intestinal e estimular a assimilação de nutrientes. São importantes estabilizadores de bactérias lácteas como bifidobactérias e lactobacillus.

Este trabalho insere-se num projeto já aprovado envolvendo várias Universidades, laboratórios e empresas de vários países da Europa. O compromisso da Universidade da Madeira é a produção de quantidades na ordem dos vários gramas de fruto-oligossacáridos (polímeros de frutose- FOS) a partir da biomassa para utilização por outras entidades parceiras.

Neste estágio, vamos usar banana verde cedida pela GESBA para otimizar métodos que serão depois aplicados a escala piloto.




PROPOSAL 2

Preparation and characterization of low generation of metallodendrimers for biomedical applications

Supervisor: João Rodrigues

Vacancies: 1

Starting date: 02/07/2018.

Working time per day: 6-7h

Schedule: Full day (starting at 09:00)

Observation and other requirements: Alunos do 2º ou 3º ano do 1º ciclo de Bioquímica (UMa), Química, Materiais ou equivalente. Estudantes do 1º ano de mestrado em Bioquímica Aplicada (UMa)/Nanoquímica e Nanomateriais ou Química/Materiais de outras Universidades. Os estagiários para além dos conhecimentos básicos de Química/Bioquímica/Materiais, devem ser fluentes em inglês, uma vez que irão trabalhar com investigadores de várias nacionalidades.

Abstract: Cancer is an important disease worldwide. According to GLOBOCAN2012, in 2012, around 14.7 million of new cancer cases and 8.6 million cancer-related deaths occurred. For that reason, different institutions are spending millions of euros trying to find new anticancer drugs.

The dendrimers (a hyperbranched type of molecules) are a topic under study at CQM for more than 10 years (see references below). This type of molecules presents potentially a significant number of biomedical uses. Mostly, they have been studied as potential nanocarriers for different kinds of drugs, such as those used in anticancer therapy. As a general goal, the student will be involved in research, involving the basic fundaments of organic and inorganic chemistry merged with biochemical studies (in cancer cells), aiming to develop a new family of metallodendrimers and evaluate their potential as active drugs against different types of cancer.

References:

  • “Dendrimers in combination with natural products and analogues as anti-cancer agents.” Mignani S.*, Rodrigues J.*, Tomas H., Zablocka M., Shi X., Caminade A.-M., Majoral J.-P*. Chem. Soc. Rev., 2018, 47, 514-532, https://doi.org/10.1039/C7CS00550D  (IF: 38.618)
  • “Divergent Route to the Preparation of Hybrid Pt-Fe 2,4,6-tris(4-ethynyl)phenyl-1,3,5-triazine Metallodendrimers for Nonlinear Optics”, Maiti S. K., Jardim M. G., Rodrigues J.*, Rissanen K., Campo J., Wenseleers W., Organometallics, 2013, 32, 406-414. http://dx.doi.org/10.1021/om300745v
  • "Gene Delivery into Mesenchymal Stem Cells: A Biomimetic Approach Using RGD Nanoclusters Based on Poly(amidoamine) Dendrimers", Pandita D., Santos J.L., Rodrigues J., Pêgo A.P., Granja P.L., Balian G., Tomás H., Biomacromolecules, 2011, 12, 724–481. http://dx.doi.org/10.1021/bm1012647
  • "Preparation and Characterization of Novel Poly(alkyliden imine) Nitrile Ruthenium Metallodendrimers", Jardim M.G., Rissanen K., Rodrigues* J., Eur. J. Inorg. Chem., 2010, 11, 1729-1735. doi: 10.1002/ejic.200901187
  • "Receptor-mediated Gene Delivery Using PAMAM Dendrimers Conjugated With Peptides Recognized by Mesenchymal Stem Cells", Santos J.L., Pandita D., Rodrigues J., Pêgo A.P., Granja P.L., Balian G., Tomás H., Mol. Pharmaceut., 2010, 7, 763–774. doi: 10.1021/mp9002877
  • "Ruthenium Metallodendrimers Based on Nitrile-Functionalized Poly(alkylidene imine)s", Catia Ornelas, Viatcheslav Vertlib, João Rodrigues* and Kari Rissanen, Eur. J. Inorg. Chem., 2006, 1, 47-50. doi:10.1002/ejic.200500799



 

PROPOSAL 3

Ligand Development by Phage Display Technology

Supervisor: Mariana Catanho da Silva Vieira

Vacancies: 1

Starting date: 02/07/2018

Working time per day: 3-4h

Schedule: 09:00-12:30

Observation and other requirements: Preference for students attending the second year or above, with some knowledge in basic molecular biology and/or microbiology. Schedule and working time are flexible and can be changed in accordance with the student.

Abstract: The aim of this internship is to develop and optimize a phage display technology. This phage display technique allows for the presentation of a high number of randomized peptide sequences on the surface of bacteriophages [1]. It is a very powerful technique with several applications, such as mapping of protein interactions, drug discovery and delivery, vaccine design and diagnostic applications. In summary, with this method, large peptide libraries can be displayed in bacteriophages and used for affinity screening of specific target molecules; and then amplified, identified and characterized. In this work, a commercially available peptide library will be used in order to identify certain target molecules.

The work plan will involve the optimization of the phage binding protocol in a control experiment using, in a first stage, streptavidin as the target. A phage library will be incubated with a target-coated surface. Phages bound to the specific target will go through several repeated cycles of incubation, elution and amplification (in a bacterial host) and re-selection: a process called biopanning. The obtained phage mixture (enriched with the relevant phage(s) with the desired binding selectivity) will be collected and subject to validation experiments. These include evaluation of the binding activity by ELISA, sequencing of the corresponding DNA and analysis of the sequence(s) via online tools (to characterize interaction and exclude target-unrelated peptides).  Several techniques will be explored and different parameters optimized in order to obtain the best outcomes: 1) preparation of media and solutions; 2) bacteria growth and maintenance by microbiology techniques; 3) coating plates with target molecules; 4) phage binding, elution and titering; 5) DNA purification for sequencing; 5) ELISA binding assay. If time permits, the same protocol will be followed and further optimized using the same phage library but a different target: an antibody specific to Zika virus that will be immobilized on a plastic surface.

The ultimate aim is to take advantage of this technique for a diagnostic application for infectious diseases, such as Dengue and Zika. There are several issues to overcome in the current diagnostic approaches. The concept of the project is to use phage display technology to identify peptides that specifically recognize antibodies for these diseases. These peptides will then serve as the basis for translation into new differential diagnostic tools for these infectious diseases, namely bacteriophague-based biosensors, where phages are used directly as diagnostic sensors.

References:

  • [1] J Bazan, I Calkosinski, A Gamian. Phage display—A powerful technique for immunotherapy. Hum Vaccin Immunother. 2012, 8: 1817-28.




PROPOSAL 4

New Insights for CdTe@dendrimers

Supervisor: Manuel Algarra

Vacancies: 2

Starting date: 02/07/2018

Working time per day: 6-7h

Schedule: 10:00 – 17:00

Observation and other requirements: n/a

Abstract: Water-soluble CdTe quantum dots have shown potential as a platform for the development of live cell imaging, but their cytotoxicity limits their biological applications. To decrease their cytotoxicity, an approach to modify CdTe quantum dots (QDs) with polyamidoamine (PAMAM) is a friendly procedure to obtain covalent bonds between them.

Our challenge is to find a cell imaging bioprobe for CQM interest, including their in vitro toxicity and their bioimaging visualization.



PROPOSAL 5

Perfil volatómico e polifenólico de produtos agroalimentares da família Allium como estratégia para identificação de compostos com potencial antiviral

Supervisor: José de Sousa Câmara

Co-supervisor: Rosa Perestrelo

Vacancies: 2

Starting date: 02/07/2018

Working time per day: 6-7h

Schedule: 09:00 – 17:30

Observation and other requirements: n/a

Abstract: Tendo por base as propriedades bioativas dos constituintes de alguns produtos agroalimentares produzidos na RAM, pretende-se neste trabalho Identificar metabolitos com potencial efeito antiviral. Será optimizada e implementada uma metodologia SPME para extração dos metabolitos voláteis combinada com a cromatografia gasosa-espectrometria de massa (GC-MS) para a identificação dos metabolitos voláteis. Será igualmente estudado o perfil de polifenóis livres de baixo peso molecular e realizada a sua quantificação por cromatografia líquida de ultra eficiência combinada com um sistema de deteção por fluorescência (UPLC-PDA).



Registration

Registration from is available here until 08th June 2018. Applicants must indicate the preferred training courses taking into account that it is not possible to repeat the training course or supervisor from the previous year(s). Consider the observation(s) and other requirements indicated for each proposal. Admittance lists will be announced from 12th june.

These training courses are free for the students of 1st Cycle of Biochemistry and 2nd Cycles of Applied Biochemistry or Nanochemistry and Nanomaterials of the University of Madeira.

For students of Biochemistry, Chemistry or similar areas from other universities the registration costs 50€. Selected candidates must have an insurance for individual accidents in order to use the laboratory areas of CQM.



Selection Process

  • 1st Priority: Students of 1st Cicle in Biochemistry who have never attended the CQM internships;
  • 2nd Priority: Students of the 2nd Cycles in Applied Biochemistry or Nanochemistry and Nanomaterials who have never attended the CQM internships;
  • 3rd Priority: Students from other universities attending Biochemistry, Chemistry or related courses;
  • 4th Priority: Other students of the 1st Cycle of Biochemistry and the 2nd Cycles in Applied Biochemistry or Nanochemistry and Nanomaterials;
  • Candidates not selected in the first or second choices will be considered substitutes.


The trainees will receive a participation certificate which will include the total worked hours.


NOTE: These internships do not replace any curricular equivalency / frequency of their courses.



PROPOSTA 1 / PROPOSAL 1

Isolamento e caracterização de polímeros de frutose bioativos

 

Supervisor: Paula Castilho

Nº de vagas/Vacancies: 2

Data de início/Starting date: 02/07/2018

Nº de horas por dia/Working time per day: 6-7

Horário/Schedule: 09h00 – 17h00

Observações e outros requisitos/Observation and other requirements: Nota mínima de 14 em Técnicas Laboratoriais Elementares e em Técnicas Laboratoriais Complementares.

 

 

Resumo/Abstract:

Isolar e caracterizar açúcares com atividade prebiótica a partir de banana verde. Estes açúcares têm efeitos benéficos para o organismo humano na redução no teor calórico do alimento, no menor risco de formação de cáries e na redução da intolerância à lactose, e também atuando no sentido de modular o sistema imune, regular o trânsito intestinal e estimular a assimilação de nutrientes. São importantes estabilizadores de bactérias lácteas como bifidobactérias e lactobacillus.

Este trabalho insere-se num projeto já aprovado envolvendo várias Universidades, laboratórios e empresas de vários países da Europa. O compromisso da Universidade da Madeira é a produção de quantidades na ordem dos vários gramas de fruto-oligossacáridos (polímeros de frutose- FOS) a partir da biomassa para utilização por outras entidades parceiras.

Neste estágio, vamos usar banana verde cedida pela GESBA para otimizar métodos que serão depois aplicados a escala piloto.

PROPOSTA 1 / PROPOSAL 1

Isolamento e caracterização de polímeros de frutose bioativos

Supervisor: Paula Castilho

Nº de vagas/Vacancies: 2

Data de início/Starting date: 02/07/2018

Nº de horas por dia/Working time per day: 6-7

Horário/Schedule: 09h00 – 17h00

Observações e outros requisitos/Observation and other requirements: Nota mínima de 14 em Técnicas Laboratoriais Elementares e em Técnicas Laboratoriais Complementares.

Resumo/Abstract:

Isolar e caracterizar açúcares com atividade prebiótica a partir de banana verde. Estes açúcares têm efeitos benéficos para o organismo humano na redução no teor calórico do alimento, no menor risco de formação de cáries e na redução da intolerância à lactose, e também atuando no sentido de modular o sistema imune, regular o trânsito intestinal e estimular a assimilação de nutrientes. São importantes estabilizadores de bactérias lácteas como bifidobactérias e lactobacillus.

Este trabalho insere-se num projeto já aprovado envolvendo várias Universidades, laboratórios e empresas de vários países da Europa. O compromisso da Universidade da Madeira é a produção de quantidades na ordem dos vários gramas de fruto-oligossacáridos (polímeros de frutose- FOS) a partir da biomassa para utilização por outras entidades parceiras.

Neste estágio, vamos usar banana verde cedida pela GESBA para otimizar métodos que serão depois aplicados a escala piloto.

 
Copyright © 2018 Centro de Química da Madeira. All Rights Reserved. - FCT ref. CHEM-Madeira-Funchal-674 | Projeto UID/QUI/00674/2013